mc3t3-e1 subclone 4 Search Results


mc3t3 e1 subclone 4  (ATCC)
99
ATCC mc3t3 e1 subclone 4
Effect of ME and magnolin on osteoblast differentiation <t>in</t> <t>MC3T3-E1</t> cells. ( A ) Cell viability, ( B , C ) cell proliferation, ( D ) ALP staining, and ( E ) ALP activity assays were performed as described in Materials and Methods. Cells were treated with ME (left panel, 25–400 μg/mL) or magnolin (right panel, 1–40 μM) for 24 h ( A , B ) and 21 days ( C ), respectively. Untreated cells were cultured in serum-free MEM alpha ( A , B ) or 10% FBS-MEM alpha ( C – E ). For the differentiation experiments, cells were cultured in differentiation medium (DM: 10% FBS-MEM alpha containing 10 mM β-glycerophosphate and 50 µg/mL ascorbic acid) and treated with ME (25–100 μg/mL) or magnolin (1–10 μM) for 14 days ( D , E ). ( D ) ALP staining images were photographed using a microscope (original magnification ×40). Results are presented as the percentage ( A – C ) or the fold-increase ( E ) of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with 10% FBS- or DM-treated cells).
Mc3t3 E1 Subclone 4, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc3t3-e1+subclone+4/pmc12985482-82-0-12?v=ATCC
Average 99 stars, based on 1 article reviews
mc3t3 e1 subclone 4 - by Bioz Stars, 2026-08
99/100 stars
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mouse pre osteoblast mc3t3 e1 cell line  (ATCC)
97
ATCC mouse pre osteoblast mc3t3 e1 cell line
Effect of ME and magnolin on osteoblast differentiation <t>in</t> <t>MC3T3-E1</t> cells. ( A ) Cell viability, ( B , C ) cell proliferation, ( D ) ALP staining, and ( E ) ALP activity assays were performed as described in Materials and Methods. Cells were treated with ME (left panel, 25–400 μg/mL) or magnolin (right panel, 1–40 μM) for 24 h ( A , B ) and 21 days ( C ), respectively. Untreated cells were cultured in serum-free MEM alpha ( A , B ) or 10% FBS-MEM alpha ( C – E ). For the differentiation experiments, cells were cultured in differentiation medium (DM: 10% FBS-MEM alpha containing 10 mM β-glycerophosphate and 50 µg/mL ascorbic acid) and treated with ME (25–100 μg/mL) or magnolin (1–10 μM) for 14 days ( D , E ). ( D ) ALP staining images were photographed using a microscope (original magnification ×40). Results are presented as the percentage ( A – C ) or the fold-increase ( E ) of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with 10% FBS- or DM-treated cells).
Mouse Pre Osteoblast Mc3t3 E1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc3t3-e1+subclone+4/pmc09080546-83-6-14?v=ATCC
Average 97 stars, based on 1 article reviews
mouse pre osteoblast mc3t3 e1 cell line - by Bioz Stars, 2026-08
97/100 stars
  Buy from Supplier
mc-3t3-e1 subclone 4  (LGC Promochem)
90
LGC Promochem mc-3t3-e1 subclone 4
Effect of ME and magnolin on osteoblast differentiation <t>in</t> <t>MC3T3-E1</t> cells. ( A ) Cell viability, ( B , C ) cell proliferation, ( D ) ALP staining, and ( E ) ALP activity assays were performed as described in Materials and Methods. Cells were treated with ME (left panel, 25–400 μg/mL) or magnolin (right panel, 1–40 μM) for 24 h ( A , B ) and 21 days ( C ), respectively. Untreated cells were cultured in serum-free MEM alpha ( A , B ) or 10% FBS-MEM alpha ( C – E ). For the differentiation experiments, cells were cultured in differentiation medium (DM: 10% FBS-MEM alpha containing 10 mM β-glycerophosphate and 50 µg/mL ascorbic acid) and treated with ME (25–100 μg/mL) or magnolin (1–10 μM) for 14 days ( D , E ). ( D ) ALP staining images were photographed using a microscope (original magnification ×40). Results are presented as the percentage ( A – C ) or the fold-increase ( E ) of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with 10% FBS- or DM-treated cells).
Mc 3t3 E1 Subclone 4, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mc3t3-e1+subclone+4/10__1007_slash_s10570___007___9182___4-43-0-7?v=LGC+Promochem
Average 90 stars, based on 1 article reviews
mc-3t3-e1 subclone 4 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Effect of ME and magnolin on osteoblast differentiation in MC3T3-E1 cells. ( A ) Cell viability, ( B , C ) cell proliferation, ( D ) ALP staining, and ( E ) ALP activity assays were performed as described in Materials and Methods. Cells were treated with ME (left panel, 25–400 μg/mL) or magnolin (right panel, 1–40 μM) for 24 h ( A , B ) and 21 days ( C ), respectively. Untreated cells were cultured in serum-free MEM alpha ( A , B ) or 10% FBS-MEM alpha ( C – E ). For the differentiation experiments, cells were cultured in differentiation medium (DM: 10% FBS-MEM alpha containing 10 mM β-glycerophosphate and 50 µg/mL ascorbic acid) and treated with ME (25–100 μg/mL) or magnolin (1–10 μM) for 14 days ( D , E ). ( D ) ALP staining images were photographed using a microscope (original magnification ×40). Results are presented as the percentage ( A – C ) or the fold-increase ( E ) of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with 10% FBS- or DM-treated cells).

Journal: International Journal of Molecular Sciences

Article Title: Novel Osteoblastogenic Activity of Magnolia kobus : The Pharmacological Potential for Osteoporosis

doi: 10.3390/ijms27052472

Figure Lengend Snippet: Effect of ME and magnolin on osteoblast differentiation in MC3T3-E1 cells. ( A ) Cell viability, ( B , C ) cell proliferation, ( D ) ALP staining, and ( E ) ALP activity assays were performed as described in Materials and Methods. Cells were treated with ME (left panel, 25–400 μg/mL) or magnolin (right panel, 1–40 μM) for 24 h ( A , B ) and 21 days ( C ), respectively. Untreated cells were cultured in serum-free MEM alpha ( A , B ) or 10% FBS-MEM alpha ( C – E ). For the differentiation experiments, cells were cultured in differentiation medium (DM: 10% FBS-MEM alpha containing 10 mM β-glycerophosphate and 50 µg/mL ascorbic acid) and treated with ME (25–100 μg/mL) or magnolin (1–10 μM) for 14 days ( D , E ). ( D ) ALP staining images were photographed using a microscope (original magnification ×40). Results are presented as the percentage ( A – C ) or the fold-increase ( E ) of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with 10% FBS- or DM-treated cells).

Article Snippet: MC3T3-E1 subclone 4 (CRL-2593) and RAW264.7 (TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Activity Assay, Cell Culture, Microscopy

Effect of ME and magnolin on mineralization in MC3T3-E1 cells. ( A ) ARS staining and ( B ) mineralization assays were performed as described in Materials and Methods. Cells were cultured in DM and treated with ME (upper panel, 25–100 μg/mL) or magnolin (lower panel, 1–10 μM) for 21 days. Cell culture medium for untreated controls was 10% FBS-MEM alpha. ( A ) ARS staining images were photographed using a microscope (original magnification ×40). Results are presented as the fold-increase of untreated controls. Statistical significance is indicated ( ### p < 0.001, compared with untreated cells; ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Journal: International Journal of Molecular Sciences

Article Title: Novel Osteoblastogenic Activity of Magnolia kobus : The Pharmacological Potential for Osteoporosis

doi: 10.3390/ijms27052472

Figure Lengend Snippet: Effect of ME and magnolin on mineralization in MC3T3-E1 cells. ( A ) ARS staining and ( B ) mineralization assays were performed as described in Materials and Methods. Cells were cultured in DM and treated with ME (upper panel, 25–100 μg/mL) or magnolin (lower panel, 1–10 μM) for 21 days. Cell culture medium for untreated controls was 10% FBS-MEM alpha. ( A ) ARS staining images were photographed using a microscope (original magnification ×40). Results are presented as the fold-increase of untreated controls. Statistical significance is indicated ( ### p < 0.001, compared with untreated cells; ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Article Snippet: MC3T3-E1 subclone 4 (CRL-2593) and RAW264.7 (TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Cell Culture, Microscopy

Effect of ME and magnolin on osteoblastogenesis-related transcription factors in MC3T3-E1 cells. Western blot analysis was performed as described in Materials and Methods. Cells were cultured in DM and treated with ( A ) ME (25–100 μg/mL) or ( B ) magnolin (1–10 μM) for 24 h. Cell culture medium for untreated controls was 10% FBS-MEM alpha. Results are presented as the fold-increase of untreated controls. Statistical significance is indicated ( ## p < 0.01, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Journal: International Journal of Molecular Sciences

Article Title: Novel Osteoblastogenic Activity of Magnolia kobus : The Pharmacological Potential for Osteoporosis

doi: 10.3390/ijms27052472

Figure Lengend Snippet: Effect of ME and magnolin on osteoblastogenesis-related transcription factors in MC3T3-E1 cells. Western blot analysis was performed as described in Materials and Methods. Cells were cultured in DM and treated with ( A ) ME (25–100 μg/mL) or ( B ) magnolin (1–10 μM) for 24 h. Cell culture medium for untreated controls was 10% FBS-MEM alpha. Results are presented as the fold-increase of untreated controls. Statistical significance is indicated ( ## p < 0.01, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Article Snippet: MC3T3-E1 subclone 4 (CRL-2593) and RAW264.7 (TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Cell Culture

Effect of ME and magnolin on osteoblastogenic markers in MC3T3-E1 cells. Quantitative PCR analysis was performed as described in Materials and Methods. Cells were cultured in DM and treated with ( A ) ME (25–100 μg/mL) or ( B ) magnolin (1–10 μM) for 7 days. Untreated cells were cultured in 10% FBS-MEM alpha. GAPDH was used as control gene. Results are shown as the fold-increase of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Journal: International Journal of Molecular Sciences

Article Title: Novel Osteoblastogenic Activity of Magnolia kobus : The Pharmacological Potential for Osteoporosis

doi: 10.3390/ijms27052472

Figure Lengend Snippet: Effect of ME and magnolin on osteoblastogenic markers in MC3T3-E1 cells. Quantitative PCR analysis was performed as described in Materials and Methods. Cells were cultured in DM and treated with ( A ) ME (25–100 μg/mL) or ( B ) magnolin (1–10 μM) for 7 days. Untreated cells were cultured in 10% FBS-MEM alpha. GAPDH was used as control gene. Results are shown as the fold-increase of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Article Snippet: MC3T3-E1 subclone 4 (CRL-2593) and RAW264.7 (TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Control

Effect of ME and magnolin on MAPK signaling pathways in MC3T3-E1 cells. Cells were cultured in DM and treated with ( A ) ME (25–100 μg/mL) or ( B ) magnolin (1–10 μM) for 15 min. Untreated cells were cultured in 10% FBS-MEM alpha. Results are shown as the fold-increase of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Journal: International Journal of Molecular Sciences

Article Title: Novel Osteoblastogenic Activity of Magnolia kobus : The Pharmacological Potential for Osteoporosis

doi: 10.3390/ijms27052472

Figure Lengend Snippet: Effect of ME and magnolin on MAPK signaling pathways in MC3T3-E1 cells. Cells were cultured in DM and treated with ( A ) ME (25–100 μg/mL) or ( B ) magnolin (1–10 μM) for 15 min. Untreated cells were cultured in 10% FBS-MEM alpha. Results are shown as the fold-increase of untreated controls. Statistical significance is indicated ( # p < 0.05, ## p < 0.01, ### p < 0.001, compared with untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with DM-treated cells).

Article Snippet: MC3T3-E1 subclone 4 (CRL-2593) and RAW264.7 (TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Protein-Protein interactions, Cell Culture

Involvement of p38 MAPK in ME- and magnolin-induced mineralization in MC3T3-E1 cells. ( A ) ARS staining and ( B ) mineralization assays were performed as described in Materials and Methods. Cells were cultured in DM and treated with ME (100 μg/mL) or magnolin (10 μM) for 21 days in the presence or absence of PD98059 (25 μM) and SB203580 (5 μM). Untreated cells were cultured in 10% FBS-MEM alpha. ( A ) ARS staining images were photographed using a microscope (original magnification ×40). Results are presented as the fold-increase of untreated controls. Statistical significance is indicated ( ### p < 0.001, compared with untreated cells; *** p < 0.001, compared with DM-treated cells).

Journal: International Journal of Molecular Sciences

Article Title: Novel Osteoblastogenic Activity of Magnolia kobus : The Pharmacological Potential for Osteoporosis

doi: 10.3390/ijms27052472

Figure Lengend Snippet: Involvement of p38 MAPK in ME- and magnolin-induced mineralization in MC3T3-E1 cells. ( A ) ARS staining and ( B ) mineralization assays were performed as described in Materials and Methods. Cells were cultured in DM and treated with ME (100 μg/mL) or magnolin (10 μM) for 21 days in the presence or absence of PD98059 (25 μM) and SB203580 (5 μM). Untreated cells were cultured in 10% FBS-MEM alpha. ( A ) ARS staining images were photographed using a microscope (original magnification ×40). Results are presented as the fold-increase of untreated controls. Statistical significance is indicated ( ### p < 0.001, compared with untreated cells; *** p < 0.001, compared with DM-treated cells).

Article Snippet: MC3T3-E1 subclone 4 (CRL-2593) and RAW264.7 (TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Cell Culture, Microscopy